TB-500

Research Use Only

All research peptides supplied by OPTMZ Peptides are intended strictly for laboratory research applications and are not designed for medical use. These materials are not approved by the U.S. Food and Drug Administration (FDA) to diagnose, treat, cure, or prevent any disease. By purchasing, the buyer confirms use strictly for scientific research purposes and not for human or animal consumption.

Product Overview

TB-500 is a research peptide associated with Thymosin Beta-4 (Tβ4), an endogenous beta-thymosin involved in actin-associated cellular processes. The submitted OPTMZ TB-500 sample was analytically identified by the testing laboratory as Thymosin β4.

Thymosin Beta-4 is investigated in controlled experimental environments for its interaction with globular actin (G-actin) and its involvement in cytoskeletal organization, cell migration, endothelial-cell biology, and actin-dependent cellular processes. Published research describes Tβ4 as a major G-actin-sequestering peptide and has examined its effects on actin polymerization and cellular migration.

Technical Specifications
Product: TB-500
Quantity: 2 mg label claim
Analyte Identified: Thymosin β4
Research Classification: Thymosin Beta-4 / Actin-Binding Research Peptide
Compound Type: Research Peptide
Primary Research Target: G-actin / actin-associated cellular pathways
CAS Number: 77591-33-4
Purity: >99.9% chromatographic purity
Identity: Thymosin β4 confirmed by HPLC-UV-MS
Measured Content: 1.83 mg
Percent of Label: 91.6%
Analysis Method: HPLC-UV-MS
Laboratory: Krause Analytical
Lab Number: 200511
Project: 145030
These specifications come directly from the independent laboratory analysis of the submitted OPTMZ TB-500 sample. The report identifies the analyte as Thymosin β4, CAS 77591-33-4, with >99.9% chromatographic purity and confirmed identity.

Analytical & Testing
Each tested batch of TB-500 undergoes analytical evaluation to verify chromatographic purity, identity, peptide content, and analytical consistency. A corresponding Certificate of Analysis is maintained for the tested lot.

For the submitted OPTMZ Peptides sample, independent testing by Krause Analytical reported:

Chromatographic Purity: >99.9%
Identity: Thymosin β4 — Confirmed
Measured Content: 1.83 mg
Label Claim: 2 mg
Percent of Label: 91.6%
Testing Method: HPLC-UV-MS
CAS Number: 77591-33-4
Laboratory: Krause Analytical
Report Issued: February 6, 2026
The laboratory states that the data represent analysis of the submitted sample and met Krause Analytical’s internal quality-assurance criteria unless otherwise flagged.

Note: Unlike several of your other COAs, this report does not contain an endotoxin result, so I would not state that this particular TB-500 lot is endotoxin tested.

Handling & Storage
TB-500 should be handled by qualified professionals in controlled laboratory environments. Appropriate storage conditions should be maintained to preserve peptide stability and structural integrity.

Avoid unnecessary exposure to heat, moisture, and repeated temperature fluctuations. Experimental preparation, storage, and handling should follow validated laboratory procedures appropriate to the intended research application.

Research Context
Thymosin Beta-4 has been investigated extensively in experimental systems involving actin biology, cytoskeletal regulation, cellular migration, endothelial biology, and cellular signaling.

Research areas include:

G-actin binding and sequestration
Actin polymerization
Cytoskeletal organization
Cell migration
Endothelial-cell biology
Cellular adhesion
Actin-dependent signaling
Angiogenesis-associated experimental models
Cellular differentiation
Actin-binding protein interactions
Cytoskeletal structure/function relationships
Thymosin Beta-4 forms complexes with actin monomers and participates in regulation of the balance between polymerized and unpolymerized actin. Experimental work in living cells has demonstrated effects on actin filament organization when cellular Tβ4 concentrations are altered.

Research has also examined Thymosin Beta-4 in endothelial-cell migration models. Studies involving human umbilical vein endothelial cells (HUVECs), for example, have evaluated migration, adhesion, tube formation, and vascular sprouting as experimental endpoints.

These areas describe experimental research contexts and do not establish therapeutic efficacy or safety in humans.

Research Application Scope
TB-500/Thymosin Beta-4 continues to be evaluated as an experimental research material for studying actin dynamics and their relationship with cellular migration, cytoskeletal organization, endothelial-cell behavior, and intracellular signaling.

Its interaction with G-actin makes Thymosin Beta-4 particularly relevant to laboratory investigations examining actin polymerization and cytoskeletal regulation. Research has additionally examined PI3K/Akt/eNOS signaling in Tβ4-associated endothelial progenitor-cell migration models.

Use Statement
For laboratory research use only. Intended strictly for in vitro and controlled experimental research applications.

Disclaimer
All products currently listed on this site are for research purposes ONLY.