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B-12 (Cyanocobalamin) Pro 10 mg research vial and box, lot tested at 99% HPLC purity, Astramide Pro Series

B-12 (Cyanocobalamin) Pro

Pro Series
Cyanocobalamin (vitamin B12), a cobalt corrinoid, 10 mg lyophilized research vial
CAS 68-19-9C₆₃H₈₈CoN₁₄O₁₄PApproximately 1355.4 g/molReagentLyophilized powder

$44.99

Ships from lot PLB-B1210-20260821-01 · 10 mg vial · 99% purity · pH Solutions Lab See the COA
Why the Pro Series costs more
  • Synthesized in the USA. Domestic ground and air only, no ocean freight.
  • Released only above 99% HPLC purity.
  • Its own lot and its own published certificate.
How the Pro Series is made and tested
Ships from Austin, TexasUnited States only
Tested by an outside labNever in-house
Every lot publishedIncluding rejected lots
Same-day dispatchOn orders before 2pm ET
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BATCH
VERIFICATION
LOT NUMBER
PLB-B1210-20260821-01
10 mg vial
IDENTITY
Retention time matched a certified reference standard
CHROMATOGRAPHIC PURITY
99%
WATER ACTIVITY
0.33 aw, pass
Analytical characterization

Every result against its specification.

Lot PLB-B1210-20260821-01 · 10 mg vial · pH Solutions Lab · reported 31 August 2026 . The bar shows where the reported result falls against Astramide’s published release criterion. The notch marks the criterion.
Within criterion Above criterion Not on this COA
Test
Method
Criterion
Result
Result vs criterion
Status
Identity
HPLC (304 nm), retention time
Match to the labelled compound
Retention time matched a certified reference standard
WITHIN SPEC
Chromatographic purity
HPLC (304 nm)
at or above 99.0%
99%
95 at or above 99.0% 100
WITHIN SPEC
Water activity
Water activity meter
below 0.65 aw
0.33 aw, pass
WITHIN SPEC
Microbial panel
qPCR
not detected
Pass, 6 organisms not detected in 1 g
WITHIN SPEC
Net content
—
Not reported on this certificate
NOT REPORTED
Endotoxin
LAL
—
Not reported on this certificate
NOT REPORTED
Sterility
—
Not reported on this certificate
NOT REPORTED
Heavy metals
—
Not reported on this certificate
NOT REPORTED
Values are reported per vial unless otherwise noted. Every figure here is taken from the batch Certificate of Analysis and nothing else.
Certificate of Analysis
B-12 (Cyanocobalamin) Pro · Lot PLB-B1210-20260821-01 · 10 mg vial · Lab No. PLB-B1210-20260821-01
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Quick reference

How to read this report

Same six keys on every Astramide product page. Only the values change.
1Lot numberMatch it to the number printed on your vial. If they differ, this report is not for your vial.
2IdentityMass spectrometry confirms the molecule is the one on the label. It conforms or it does not. There is no partial result.
3Chromatographic purityThe share of the material that is the target peptide. A purity figure quoted without a method is not a purity figure.
4Net contentHow much peptide is in the vial, as measured by the laboratory. A different question from purity.
5EndotoxinMeasured against a ceiling, not a floor. Sensitivity differs by laboratory, so results are not compared across labs.
6Laboratory, method, dateWho ran it, on what instrument, and when. A report with no named laboratory or no date is not a report.
Molecular profile

B-12 (Cyanocobalamin) Pro, in structural terms.

B-12 (Cyanocobalamin) Pro molecular structure
Sequence
Not applicable. Cyanocobalamin is a cobalt corrinoid, not a peptide, and has no amino acid sequence.
Molecular formula
C₆₃H₈₈CoN₁₄O₁₄P
Molecular weight
Approximately 1355.4 g/mol
CAS number
68-19-9
Structure type
Reagent
Parent sequence
Not applicable. Cyanocobalamin is a cobalt-containing corrinoid, not a peptide or a fragment of one.
Primary molecular target
Cyanocobalamin has no receptor target. In published biochemical work it is studied as a precursor of the cobalamin cofactors used by methionine synthase and methylmalonyl-CoA mutase, as a substrate of the trafficking chaperone CblC, and as a ligand of the binding proteins intrinsic factor, transcobalamin and haptocorrin.
Physical form
Lyophilized powder
PubChem CID
165339223

Cyanocobalamin is the cyanide form of vitamin B12. It is built on a corrin ring, a macrocycle of four reduced pyrrole rings, with a cobalt(III) ion at its center. Below the ring, the cobalt is coordinated by a 5,6-dimethylbenzimidazole nucleotide tail; above it, by a cyanide ligand. It is not a peptide. Its formula is C63H88CoN14O14P and its molecular weight is about 1355.4 g/mol.

Research profile

Where B-12 (Cyanocobalamin) Pro appears in the literature.

Biochemistry; Enzymology; Cofactor Biology; Protein-Ligand Binding; Analytical Chemistry
Research applications
  • Published studies have used cyanocobalamin in:
  • Cobalamin-dependent enzyme catalysis studies
  • Cofactor processing and decyanation studies
  • Binding kinetics with cobalamin transport proteins
  • Reference standard work for cobalamin analysis
Experimental systems
  • Purified recombinant proteins
  • Isolated enzyme preparations
  • Cell-free spectroscopic assays
  • Analytical reference standard preparations
Specific assays
  • UV-visible absorption spectroscopy of cobalamin species
  • Stopped-flow and fluorescence binding kinetics
  • Enzyme activity assays
  • HPLC for purity and identity
Measured endpoints
  • Conversion of cyanocobalamin to other cobalamin forms
  • Binding and dissociation rate constants
  • Enzyme turnover with cobalamin cofactors
  • Chromatographic purity
Mechanism and research context

What has been studied, and in what systems.

Cyanocobalamin is the most widely used form of vitamin B12 in the laboratory, valued for its stability as a crystalline solid.

A review of cobalamin-dependent enzymes describes how the cofactor forms methylcobalamin and adenosylcobalamin support catalysis by methionine synthase and methylmalonyl-CoA mutase (Banerjee and Ragsdale, 2003).

Before cyanocobalamin can serve as a cofactor precursor, its cyanide ligand must be removed. Biochemical work showed that the trafficking chaperone CblC catalyzes this decyanation in vitro (Kim et al., 2008). Separate binding studies compared the kinetics of cobalamin binding to intrinsic factor, transcobalamin and haptocorrin (Fedosov et al., 2002). These studies place cyanocobalamin in cofactor biochemistry and protein-ligand binding research.

These are published research systems. They do not describe approved clinical applications, and they do not establish efficacy or safety in humans.

Cited literature

Banerjee R, Ragsdale SW, 2003. The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes. Annu Rev Biochem. PMID 14527323. https://pubmed.ncbi.nlm.nih.gov/14527323/
Kim J, Gherasim C, Banerjee R, 2008. Decyanation of vitamin B12 by a trafficking chaperone. Proc Natl Acad Sci U S A. PMID 18779575. https://pubmed.ncbi.nlm.nih.gov/18779575/
Fedosov SN, Berglund L, Fedosova NU, Nexo E, 2002. Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, transcobalamin, and haptocorrin. J Biol Chem. PMID 11788601. https://pubmed.ncbi.nlm.nih.gov/11788601/

Evidence level
The structure and chemistry of cyanocobalamin are well established, and its processing and protein binding are documented in biochemical studies with purified proteins. Statements on this page describe experimental systems, not outcomes in humans.
Handling and storage
As supplied
Lyophilized powder in a sealed vial. Held in laboratory-rated freezers from arrival to shipment.
Storage
Store per the conditions printed on the vial label. Avoid heat, moisture and repeated temperature cycling.
Handling
For qualified professionals in controlled laboratory environments, following validated laboratory procedures.
Frequently asked questions

About this compound and its batch data.

What is cyanocobalamin?

Cyanocobalamin is the cyanide form of vitamin B12, a cobalt-containing corrinoid. Its formula is C63H88CoN14O14P, its molecular weight is about 1355.4 g/mol and its CAS number is 68-19-9. B-12 (Cyanocobalamin) Pro is supplied as a lyophilized powder in a 10 mg vial. Sold for research use only.

Is B-12 (cyanocobalamin) a peptide?

No. Cyanocobalamin is built on a corrin ring with a central cobalt ion, a nucleotide tail and a cyanide ligand, not a chain of amino acids, so it has no amino acid sequence. It is listed in the Pro Series because each lot is released under its own certificate with the same 99% HPLC purity criterion applied to the rest of the series.

What has cyanocobalamin been examined for in published research?

A review describes how cobalamin cofactors support catalysis by methionine synthase and methylmalonyl-CoA mutase (Banerjee and Ragsdale, 2003, PMID 14527323). The chaperone CblC removes the cyanide ligand in vitro (Kim et al., 2008, PMID 18779575), and binding kinetics with transport proteins have been compared (Fedosov et al., 2002, PMID 11788601). Astramide makes no claim about outcomes in humans.

What purity does the current B-12 Pro lot reach?

Lot PLB-B1210-20260821-01 returned 99% purity by HPLC at 304 nm, reported by pH Solutions Lab on 31 August 2026 (lab sample ID 260827-899-NHS-2). Within the same run, retention time matched a certified reference standard. The lot meets the Pro Series release criterion of at or above 99% HPLC purity. The certificate is published on this page.

How much cyanocobalamin is in the vial?

The label claim is 10 mg per vial, and the certificate lists a target fill of 10.00 mg per vial across 300 vials in the lot. Net content was not separately measured on this certificate, so Astramide does not publish a measured figure for it. Purity and net content are separate measurements.

How should cyanocobalamin be stored for research use?

Lyophilized cyanocobalamin is stored at -20°C and protected from light, because cobalamins are light-sensitive and can convert to other cobalamin forms on exposure. Once reconstituted, the solution is held at 2 to 8°C, protected from light, and used within 14 to 30 days. Repeated temperature cycling and moisture should be avoided.

How is Astramide's B-12 Pro tested?

Lot PLB-B1210-20260821-01 was tested by pH Solutions Lab, an independent ISO/IEC 17025:2017 accredited laboratory (PJLA #84351). The certificate reports purity by HPLC at 304 nm, a qPCR microbial panel of six organisms (none detected) and water activity of 0.33 aw. Net content, endotoxin, sterility and heavy metals are not reported. Results apply only to this lot.

What does Pro Series mean for B-12 (Cyanocobalamin) Pro?

B-12 (Cyanocobalamin) Pro is part of the Astramide Pro Series. It is American-made, synthesized in the USA, and moves by domestic ground and air only, with no ocean freight. It is released under its own lot, and only when HPLC purity measures at or above 99%. The full certificate for each lot is published on this page.

Research use only

This product is intended strictly for in-vitro laboratory research conducted by qualified professionals. It is not intended for human or animal consumption, clinical use, or therapeutic application. The statements on this page have not been reviewed by the FDA. These research peptides are not intended to diagnose, treat, cure, or prevent any disease. Research Use Only.

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