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NAD+ Pro research vial and box
NAD+ Pro
  1. NAD+ Pro research vial and box
  2. NAD+ Pro

NAD+ Pro

Pro Series
NAD+ (nicotinamide adenine dinucleotide), an endogenous redox coenzyme
CAS 53-84-9C₂₁H₂₇N₇O₁₄P₂Approximately 663.4 g/molReagentLyophilized powder

Price range: $79.00 through $1,159.00

Ships from lot NHS-NAD500-20260903-01 · 500 mg vial · 99% purity · pH Solutions Lab See the COA
Why the Pro Series costs more
  • Synthesized in the USA. Domestic ground and air only, no ocean freight.
  • Released only above 99% HPLC purity.
  • Its own lot and its own published certificate.
How the Pro Series is made and tested
Ships from Austin, TexasUnited States only
Tested by an outside labNever in-house
Every lot publishedIncluding rejected lots
Same-day dispatchOn orders before 2pm ET
SKU: N/A Category: Tags: ,
Batch history

Every lot we have tested.

2 lots, including any we rejected. Select one to see its report.
BATCH
VERIFICATION
LOT NUMBER
NHS-NAD500-20260903-01
500 mg vial
IDENTITY
Match (C21H27N7O14P2, 663.4 g/mol)
CHROMATOGRAPHIC PURITY
99%
NET CONTENT
509.305 mg (101.861% of 500 mg label claim)
Analytical characterization

Every result against its specification.

Lot NHS-NAD500-20260903-01 · 500 mg vial · pH Solutions Lab · reported 29 September 2026 . The bar shows where the reported result falls against Astramide’s published release criterion. The notch marks the criterion.
Within criterion Above criterion Not on this COA
Test
Method
Criterion
Result
Result vs criterion
Status
Identity
Match to the labelled compound
Match (C21H27N7O14P2, 663.4 g/mol)
WITHIN SPEC
Chromatographic purity
HPLC (254 nm)
at or above 99.0%
99%
95 at or above 99.0% 100
WITHIN SPEC
Net content
509.305 mg (101.861% of 500 mg label claim)
80 120
REPORTED
Appearance
Visual
white lyophilized powder
White to off-white lyophilized powder cake, free of visible particulates
WITHIN SPEC
Water content
Karl Fischer
reported
0.0%
WITHIN SPEC
pH
reported
7.0
WITHIN SPEC
Water activity
within laboratory limit
0.23 aw, pass
WITHIN SPEC
Microbial panel
not detected
Pass, 10 organisms and counts not detected in 1 g
WITHIN SPEC
Heavy metals
within laboratory limits
Pass. As, Cd, Pb, Hg each 0.000 ppm
WITHIN SPEC
Residual solvents
within laboratory limits
Pass, 12 solvents each 0.000 ppm
WITHIN SPEC
Sterility
sterile
Pass
WITHIN SPEC
Mycotoxins
within laboratory limits
Pass
WITHIN SPEC
Endotoxin
LAL
—
Not reported on this certificate
NOT REPORTED
Values are reported per vial unless otherwise noted. Every figure here is taken from the batch Certificate of Analysis and nothing else.
Certificate of Analysis
NAD+ Pro · Lot NHS-NAD500-20260903-01 · 500 mg vial · Lab No. NHS-NAD500-20260903-01
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Quick reference

How to read this report

Same six keys on every Astramide product page. Only the values change.
1Lot numberMatch it to the number printed on your vial. If they differ, this report is not for your vial.
2IdentityMass spectrometry confirms the molecule is the one on the label. It conforms or it does not. There is no partial result.
3Chromatographic purityThe share of the material that is the target peptide. A purity figure quoted without a method is not a purity figure.
4Net contentHow much peptide is in the vial, as measured by the laboratory. A different question from purity.
5EndotoxinMeasured against a ceiling, not a floor. Sensitivity differs by laboratory, so results are not compared across labs.
6Laboratory, method, dateWho ran it, on what instrument, and when. A report with no named laboratory or no date is not a report.
Molecular profile

NAD+ Pro, in structural terms.

NAD+ Pro molecular structure
Sequence
Not applicable. NAD+ is a dinucleotide coenzyme, not a peptide.
Molecular formula
C₂₁H₂₇N₇O₁₄P₂
Molecular weight
Approximately 663.4 g/mol
CAS number
53-84-9
Structure type
Reagent
Primary molecular target
NAD+-dependent dehydrogenases (as a redox cofactor); sirtuin deacetylases (SIRT1-SIRT7), poly(ADP-ribose) polymerases (PARPs) and CD38 (as a consumed substrate).
Physical form
Lyophilized powder
PubChem CID
5892

NAD+ is a dinucleotide, not a peptide. It consists of nicotinamide mononucleotide and adenosine monophosphate joined through a pyrophosphate bridge. In cells it cycles between the oxidized form (NAD+) and the reduced form (NADH), and it is also consumed as a substrate by enzymes that transfer its ADP-ribose group.

Research profile

Where NAD+ Pro appears in the literature.

Biochemistry; Enzymology; Cell Biology; Bioenergetics; Redox Biology
Research applications
  • Published studies have used NAD+ in:
  • Enzyme kinetics of NAD+-dependent dehydrogenases
  • Sirtuin deacetylase activity assays
  • PARP activity and ADP-ribosylation studies
  • NAD+/NADH ratio and NAD+ salvage pathway studies
  • Mitochondrial and nuclear NAD+ pool studies
Experimental systems
  • Purified recombinant enzymes
  • Yeast models (Sir2 studies)
  • Cultured mammalian cell lines
  • Isolated mitochondria
  • Cell-free coupled enzyme assays
Specific assays
  • Spectrophotometric NADH measurement at 340 nm
  • Enzymatic cycling assays
  • LC-MS/MS quantification of NAD+ metabolites
  • Fluorometric sirtuin deacetylase assays
  • PARP activity assays
Measured endpoints
  • NAD+ and NADH concentrations and their ratio
  • Enzyme turnover and Michaelis constants for NAD+
  • Sirtuin deacetylation rate
  • ADP-ribose polymer formation
  • NAD+ salvage pathway enzyme activity
Pcs

Single Vial, Pack of 10
Strength

500mg, 1000mg
Mechanism and research context

What has been studied, and in what systems.

NAD+ acts in two distinct ways in experimental systems: as a redox cofactor that cycles between NAD+ and NADH, and as a substrate that enzymes consume. Studies of the yeast protein Sir2 showed that it is an NAD-dependent histone deacetylase, which linked NAD+ availability to enzymatic gene silencing (Imai et al., 2000).

Later work showed that the NAD+ salvage pathway is regulated by the CLOCK-SIRT1 circadian machinery in cell and mouse models (Nakahata et al., 2009).

Reviews have described how NAD+ is distributed between mitochondrial and nuclear pools and how consuming enzymes such as sirtuins, PARPs and CD38 compete for it (Cantó et al., 2015). These studies place NAD+ in enzymology and redox biology research.

These are published research systems. They do not describe approved clinical applications, and they do not establish efficacy or safety in humans.

Cited literature

Imai S et al., 2000. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature. PMID 10693811. https://pubmed.ncbi.nlm.nih.gov/10693811/
Nakahata Y et al., 2009. Circadian control of the NAD+ salvage pathway by CLOCK-SIRT1. Science. PMID 19286518. https://pubmed.ncbi.nlm.nih.gov/19286518/
Cantó C et al., 2015. NAD(+) Metabolism and the Control of Energy Homeostasis: A Balancing Act between Mitochondria and the Nucleus. Cell Metab. PMID 26118927. https://pubmed.ncbi.nlm.nih.gov/26118927/

Evidence level
NAD+ is a well-characterised endogenous coenzyme with an extensive biochemical and enzymological literature. Statements on this page describe its function in experimental systems, not outcomes in humans.
Handling and storage
As supplied
Lyophilized powder in a sealed vial. Held in laboratory-rated freezers from arrival to shipment.
Storage
Store per the conditions printed on the vial label. Avoid heat, moisture and repeated temperature cycling.
Handling
For qualified professionals in controlled laboratory environments, following validated laboratory procedures.
Frequently asked questions

About this compound and its batch data.

What is NAD+?

NAD+ (nicotinamide adenine dinucleotide) is an endogenous coenzyme made of nicotinamide mononucleotide and adenosine monophosphate joined by a pyrophosphate bridge. Its formula is C21H27N7O14P2, its molecular weight is about 663.4 g/mol and its CAS number is 53-84-9. NAD+ Pro is supplied as a lyophilized powder in 500 mg and 1000 mg vials. Sold for research use only.

What has NAD+ been examined for in published research?

NAD+ has been studied as a redox cofactor for dehydrogenase enzymes and as a substrate consumed by sirtuins, PARPs and CD38. Studies showed that the yeast protein Sir2 is an NAD-dependent deacetylase (Imai et al., 2000, PMID 10693811), and that the NAD+ salvage pathway is under circadian control (Nakahata et al., 2009, PMID 19286518). Astramide makes no claim about outcomes in humans.

What purity do the current NAD+ Pro lots reach?

Both lots returned 99% chromatographic purity by HPLC at 254 nm, reported by pH Solutions Lab on 29 September 2026. The 500 mg lot is NHS-NAD500-20260903-01 (certificate 260917-899-NHS-3) and the 1000 mg lot is NHS-NAD1000-20260823-01 (certificate 260902-899-NHS-3). Identity was confirmed for both. The full certificates are published on this page.

How much NAD+ is in each vial size?

Each vial size has its own lot and certificate. The 500 mg lot measured 509.305 mg per vial, which is 101.861% of the label claim. The 1000 mg lot measured 997.76 mg per vial, which is 99.776% of the label claim. Astramide publishes each measured figure exactly as the laboratory reports it, above or below the label.

How should NAD+ be stored for research use?

Lyophilized NAD+ is stored at -20°C and protected from light and moisture. Once reconstituted, the solution is held at 2 to 8°C and used promptly, because NAD+ in solution degrades faster than the dry powder. Repeated temperature cycling should be avoided, because it can affect stability and the comparability of experimental results.

Is NAD+ a peptide?

No. NAD+ is a dinucleotide coenzyme, not a peptide, and it has no amino acid sequence. It is made of two nucleotides, nicotinamide mononucleotide and adenosine monophosphate, linked through their phosphate groups. Astramide lists it alongside research peptides because it is used in related cellular and enzymology research.

How is Astramide's NAD+ Pro tested?

Both lots were tested by pH Solutions Lab, an independent ISO/IEC 17025:2017 accredited laboratory (A2LA R243, PJLA Testing #84351). Each certificate reports identity, chromatographic purity at 254 nm, net content, water content, pH, water activity, a 10-organism microbial panel, heavy metals, residual solvents, sterility and mycotoxins. Results apply only to the lot tested.

What is the difference between NAD+ and NAD+ Pro?

NAD+ Pro is part of the Astramide Pro Series. It is American-made, synthesized in the USA, and moves by domestic ground and air only, with no ocean freight. Each vial size is released under its own lot, and only when HPLC purity measures at or above 99%. The full certificate for each lot is published on this page. The standard NAD+ is released under separate lots with separate certificates.

Research use only

This product is intended strictly for in-vitro laboratory research conducted by qualified professionals. It is not intended for human or animal consumption, clinical use, or therapeutic application. The statements on this page have not been reviewed by the FDA. These research peptides are not intended to diagnose, treat, cure, or prevent any disease. Research Use Only.

COA library Every lot we have tested Search by batch number. Past lots stay published. How we test The analytical panel What each method measures and the criterion each lot must meet. Open Lab Send us your peptides Any vendor. Same lab. Your own Certificate of Analysis.
Ships from Austin, TexasUSPS or UPS, your choice
United States onlyFree shipping at $150 and over
Optional shipping insuranceAdded at checkout
Replacement or store creditOn approved claims. See refund policy.

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